Introduction+to+Radioimmunoassay

Developed by
Center for Radiochemistry and Nuclear Materials Department of Chemistry Loughborough University

Learning Goals
To understand the technique of radioimmunoassay by performing a chemical ‘mock-up’ of the procedure.

Theory
Theoretical background for radioimmunoassay

Experimental Procedure
Experimental procedure for Introduction to Radioimmunoassay

Questions for the Students
REPORTING

Other
1. Which was the plot that gave the most sensitive answer? 2. What is the major source of error in this experiment? 3. What are the other minor sources of error?

Safety Aspects
Radioactive iodine is potentially very toxic if ingested. Although amounts of activity in this experiment are very low, disposable gloves and eye protection should be worn to avoid direct contact. Under no circumstances should the 125 I be used under oxidizing conditions when elemental iodine might be formed with a danger of inhalation.

Equipment
Triathler Waste Bucket 1 mL autopipette Centrifuge Marker pen

1 mL of 5 different antigen solutions 1 mL assay solution 14 gamma vials+lids 1 mL tips 10 mL buffer pH 6.9 1 mL antibody times 6
 * Consumables **

Preparation for the lab Supervisor
The preparation that the lab Supervisor needs to do to ready the lab.

Feedback from Users and Supervisors
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